Subsequently, we employed small interfering RNAs (siRNAs) to knockdown these aHBGs and observed significant suppression of ESCC cell proliferation, with NKX2‐5, LHX1, DLX1, GBX2, and TLX3 exhibiting the most pronounced inhibitory effects (Figure3A). Here, TLX3 is linked to esophageal squamous cell carcinoma.