Conversely, KLF5-overexpressing cells exhibited an increase in the volume and number of spheres formed (SupFig. 2A and SupFig. 2D).Flow cytometry results indicated a significant increase in the proportion of ALDH positive cells in ovarian cancer cell lines that overexpressed KLF5 (SupFig. 2B and SupFig. 2E). Here, KLF5 is linked to ovarian carcinoma.