Conversely, KLF5-overexpressing cells exhibited an increase in the volume and number of spheres formed (SupFig. 2A and SupFig. 2D).Flow cytometry results indicated a significant increase in the proportion of ALDH positive cells in ovarian cancer cell lines that overexpressed KLF5 (SupFig. 2B and SupFig. 2E). This evidence concerns the gene LDHA and ovarian cancer.