Among the 12 HCC EV-specific gene candidates, 6 genes were differentially expressed (P < 0.05, Fig. 5B), which were identified as ALB, APOH, FGB, FGG, H2AX, and TF. We summarized the relative intensities of these 6 HCC EV-specific genes across the 70 patients in heatmaps (Fig. 5C). This evidence concerns the gene H2AX and hepatocellular carcinoma.