To further validate that HINT2-regulated proteins are associated with SIRT3, we performed co-IP assays and found that the overexpression of HINT2 significantly decreased the acetylation levels of SDHA and IDH2 in HepG2 cells (Fig. 5d), whereas the knockout of Hint2 increased the acetylation levels of PDHA1, IDH2 and SDHA in the livers of both SCD- and HFD-fed mice (Fig. 5e). The gene discussed is PDHA1; the disease is Schnyder corneal dystrophy.