To further elucidate the regulatory role of Zbp1-mediated PANoptosis in P. gingivalis-induced periodontitis, Zbp1-knockout mice (mZbp1KO, Zbp1−/−) mice were generated and IHC staining confirmed the deficiency of Zbp1 in the periodontal tissues (Fig. 5a). This evidence concerns the gene ZBP1 and periodontitis.