Among the m6A-modified ALS risk genes (34 out of 81 genes in Supplementary Data 2), expression of two genes—Fus and Bscl2—was significantly reduced, whereas that of PIKfyve was increased in the skeletal MNs of ChAT-Cre; Mettl14floxed mice (Fig. 6g, h), in accordance with dysregulated ALS risk genes identified from patients48. This evidence concerns the gene BSCL2 and amyotrophic lateral sclerosis.