Finally, we further verified several newly identified m6A-modified sites in ALS risk genes, including Dctn1, Epha4, C9orf72, Glt8d1, Cacna1h, Chrna3, Bscl2, Fig4, Hnrnpa2b1, Ubqln2, Hnrnpa1, Tuba4a, Sod1, Chmp2b, and PIKfyve (Supplementary Fig. 6b). Here, PIKFYVE is linked to amyotrophic lateral sclerosis.