Consistent with the subcutaneous model, IHC staining of liver tumors showed decreased CD206 expression, increased iNOS expression, elevated CD8+ T cell infiltration, and reduced PD‐1 expression (Figure 5F), suggesting that NUPR1 inhibition may restore a more immunostimulatory microenvironment conducive to tumor cell eradication. This evidence concerns the gene NOS2 and neoplasm.