To explore potential mechanisms underlying CXCL6‐mediated regulate proliferation and migration in CCA, RNA‐Seq was performed on sh‐NC and sh‐CXCL6 HuCCT1 cells, identifying 233 significantly upregulated and 171 significantly downregulated genes (with q‐value ≤ 0.05 and log|FC| ≥ 1; Figure 3D). Here, CXCL6 is linked to cholangiocarcinoma.