AKT1 and cholangiocarcinoma: The JAK‐STAT and PI3K‐AKT pathways regulate biological processes in tumor cells in various ways.[48, 49] Notably, the JAK‐STAT pathway was shown to boost de novo fatty acid synthesis and fatty acid uptake in the TIME,[48, 50] while PI3K‐AKT activation can cause lipid peroxidation in gastric cancer.[51] To explore these possibilities in CCA, we applied GO‐BP (Gene Ontology – Biological Processes) analysis to the RNA‐Seq data mentioned above and noted that lipid metabolism‐related pathways were most highly enriched (Figure5A).