This study aims to explore, using molecular biology techniques, whether a potential functional relationship exists between ARPC3/ARPC4 and HSP70 in LPS-induced mastitis and to elucidate the roles of ARPC3/ARPC4 and HSP70 in MAC-T cell apoptosis, providing insights into new therapeutic targets for mastitis. The gene discussed is HSPA1A; the disease is mastitis.