The extent of disease alleviation inversely correlated to local NO production and production of inflammatory cytokines (TNFα, IL-12, and IL-1β), while it positively correlated with the activity of antioxidative enzymes (CAT, SOD, and GST) and the production of anti-inflammatory cytokines (TGFβ and IL-10) in the colon, providing further evidence for the protective role of rBanLec in the oxidative environment of colitis. The gene discussed is TNF; the disease is colitis.