PNRC1 and melanoma: After quality control, based on unbiased clustering and spot features, spots were classified as 3 clusters of melanoma cells, E/M Status with high expression of Espn, Mpp2, Ndrg1 and Fosl2, erythrocytes with high expression of Hba-a2, Hbb-bs and Hbb-bt, epithelial cells with high expression of Wee1, Nr2f6 and Pnrc1, monocytes/T cells with high expression of Il1b, G0s2, Cxcl3, Cd14 and Ccl4, fascia cells with high expression of Col3a1 or keratinocyte with high expression of Krt5 and Krt14 (Figure 6B).