To further investigate whether SLCs can be presented to T cells by DCs so that T cells can kill B16F10 melanoma cells, we extracted BMDCs from mouse bone marrow and CD8+ T cells from mouse spleen, and then designed a co-culture experiment with B16F10-GFP cells as shown in Figure 3D. After co-culture, the GFP signal is analyzed by flow cytometry, which can reflect the proportion of tumor cell survival. The gene discussed is CD8A; the disease is melanoma.