After quality control, based on unbiased clustering and spot features, spots were classified as 3 clusters of melanoma cells, E/M Status with high expression of Espn, Mpp2, Ndrg1 and Fosl2, erythrocytes with high expression of Hba-a2, Hbb-bs and Hbb-bt, epithelial cells with high expression of Wee1, Nr2f6 and Pnrc1, monocytes/T cells with high expression of Il1b, G0s2, Cxcl3, Cd14 and Ccl4, fascia cells with high expression of Col3a1 or keratinocyte with high expression of Krt5 and Krt14 (Figure 6B). This evidence concerns the gene ESPN and melanoma.