The MIA analysis showed that metastatic dissemination cells, Treg, and Tex cells were enriched in both the tumor and interface regions of PT tissues, while cytotoxic CD8 (CCL5) and CD4 (GZMK) cells were absent from these regions (Figure 4G), suggesting an immune-suppressive microenvironment at the tumor interface. This evidence concerns the gene CD8A and neoplasm.