Multifluorescent staining of pancreatic cancer tissues was conducted using antibodies against CD4 (white), CD8 (light blue), CD20 (pink), FOXP3 (red), VIM (green), and panCK (yellow), along with 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Figure S6F). The gene discussed is FOXP3; the disease is pancreatic neoplasm.