To investigate the relevance of podocyte-derived EVs to glomerulosclerosis in vivo, we carried out animal studies using Ang II/ADR model by injection of the Ang II-EVs isolated from Ang II-treated MPC5 cells, as shown in Figure 6A. Ang II/ADR mice were given same amounts of Ctrl-EVs or Ang II-EVs every two days from the third week after Ang II infusion (Figure 6A). Here, AGT is linked to glomerulosclerosis.