Western blot analysis revealed that Fer-1 treatment effectively blocked the activation of ferroptosis pathway (Figure 1K-L) and reduced the protein levels of fibrotic markers and heart failure indicators, such as collagen type III alpha 1 chain (COL3A1), fibronectin (Fn), transforming growth factor beta 1 (TGFβ1), atrial natriuretic peptide (ANP), and b-type natriuretic peptide (BNP) (Figure 1K-L) in DOX-treated mouse hearts. This evidence concerns the gene TGFB1 and heart failure.