Apoptosis increased significantly as well, which was manifested by apoptotic cell death, as assessed using TUNEL staining (Figure 3G) and the expression of apoptosis-related proteins Bax, Bcl-2, Bcl-2/Bax, and active-caspase 3 using WB analyses and immunofluorescence (Figure 3H-M) in the AKI group. This evidence concerns the gene BAX and acute kidney injury.