To determine the neutralization activity of the 22 sVNT-positive pandemic samples with RBD- ACE2 blocking properties to prevent infection, we generated lentiviral particles pseudotyped with the spike proteins of the SARS-CoV-2 Wuhan-Hu-1 D614G, Delta (B.1.617.2), and Omicron (B.1.1.529) variants. This evidence concerns the gene ACE2 and infection.