MRC1 and colon adenocarcinoma: To explore the characteristics of CSC-related clusters, immune infiltration levels of 22 immune cells among the 4 subtypes in COAD were obtained from known studies and shown in Figure 2A. Moreover, clinical characteristics including the number of lymph nodes, whether MMR is deficient (dMMR), MSI statues treatment statues, pathological stage, TNM stage, age, and gender were interrogated among different subtypes (Figure 2B).