Interestingly, we found that n-3 PUFAs did not significantly increase GLUT4 expression in all cases, such as DHA-treated C2C12 myotubes and DHA- and EPA-treated male mice, which further suggested that n-3 PUFAs alleviated hyperglycemia by promoting GLUT4 translocation rather than depending on increased GLUT4 expression. Here, SLC2A4 is linked to Hyperglycemia.