In agreement, in the present study, n-3 PUFA supplementation accelerated glycolysis and TCA cycle by advancing fructose 6-phosphate, malic acid, and fumaric acid in db/db mice, while the declined pyruvate levels, which were also detected in people with prediabetes, suggested elevating the PDH-mediated process from glycolysis to TCA cycle. Here, PDP1 is linked to prediabetes syndrome.