In order to infer fate trajectories of these different phenotypes that were clonally linked by a common TCR barcode, pseudotime analysis by Monocle was carried out specifically on cells with tumor-blood-matched TCRs from GZMB+, GZMK+, CXCL13+, Tregs, Prolif, and MAIT populations (Figure 3B). This evidence concerns the gene GZMB and neoplasm.