Here, we performed transduction of LOs differentiated from hiPSC from a healthy donor (wt/wt) (LOs-WT) and from a cystic fibrosis patient with homozygous F508del mutation of the CFTR gene (F508del/F508del) (LOs-CF) by recombinant viral vectors with tropism to human respiratory cells—rAdV serotype 5 and rAAVs vectors serotypes 5, 6 and 9—with genes of fluorescent reporter. This evidence concerns the gene CFTR and cystic fibrosis.