Secondary BAs found to be differentially abundant in CLL patient plasma (UDCA, 7KLCA, HDCA, MDCA, IDCA) slightly increased PD-L1 expression on CLL cells, while common, but not differential, BAs (CDCA, TCA, TCDCA, DCA, TLCA, TDCA) slightly increased LAG3 expression on CLL cells (Figure 4A). The gene discussed is CD274; the disease is B-cell chronic lymphocytic leukemia.