Western blot analysis also showed that the expression level of the CENPF protein was higher in melanoma tissues than that in adjacent tumor tissues, and representative images are shown in Figure 2D. In addition, we investigated the expression of CENPF in a TMA containing 128 melanoma tissue samples and found CENPF was indeed upregulated in most of the tumors compared with peritumor tissues (Figure 2F). Here, CENPF is linked to neoplasm.