Through LASSO regression, 15 DEEGs (AGRN, EXPH5, CLIC4, TSPAN6, TGFBR3, FN1, MMP9, KRT13, THY1, HBA1, FCGBP, PFN2, BST2, EPCAM, and PIP) were identified as critical markers for predicting HNSCC development. The gene discussed is TGFBR3; the disease is head and neck squamous cell carcinoma.