iNOS–/–vs. WT:(mostly) anti-oxidative: ↓ MDA, ↑ GPX, ↓ NOX-4, ↑ pNRF2, ↔ SOD2, ↑ HO1 in hearts↓ cardiac dysfunction: ↑ cardiac diastolic function (↓ mitral E/E’ ratio)↓ pulmonary congestion: ↓ lung edema (wet weight/dry weight ratio)other effects: ↑ running distance, ↔ heart weight/tibia length ratio, ↔ HR, ↔ ejection fraction, ↔ systolic BP, ↔ diastolic BP. This evidence concerns the gene NOX4 and pulmonary vascular congestion.