Supplementary Figure 7 shows human and bacterial urine EV proteins with the greatest variation among clusters. In Cluster 1, patients with long PFS, subsequent line ICI, low PD-L1 expression, and female gender were overrepresented, whereas Cluster 2 consisted of patients with long PFS and no COPD. Also, patients with first-line ICI-treatment and CHT-naivety were overrepresented in this cluster. Cluster 3 included the highest number of patients with short PFS, where male gender and high BMI (>30 kg/m2) were also overrepresented (Figure 5C). Here, CD274 is linked to chronic obstructive pulmonary disease.