After adjusting for confounding factors, CEA (Odd ratio (OR):2.11; 95% confidence interval (CI): 1.17-3.81), SII (OR:1.02; 95%CI: 1.01-1.04), tumor diameter (OR:2.43; 95%CI: 1.23-4.79)were identified as significant risk factors that affect lymph node metastasis, while PNI (OR:0.90; 95%CI: 0.85-0.96) was associated with a lower risk for lymph node metastasis in GC. This evidence concerns the gene CEACAM5 and neoplasm.