To further analysis the colocalization rate ratios, we found that the ratios of isolectin B4 (IB4)/NF200, calcitonin gene related peptide (CGRP)/NF200 and tropomyosin receptor kinase B (TrkB)/NF200 were significantly higher in the FMT-IBS group compared to the FMT-control group (Figure 3(f)), indicating that more pain-related neurons colocalized with Piezo2. The gene discussed is PIEZO2; the disease is irritable bowel syndrome.