Here, we demonstrate that even though SARS-CoV-2 Delta can use Jamaican fruit bat ACE2 for cell entry to a higher degree than SARS-CoV-2 Lineage A, the species is unable to support robust in vivo infection after a combination of oral and intranasal inoculation; we observed no seroconversion, no shedding, no clinical signs, and infectious virus in only one lung sample on day 1. This evidence concerns the gene ACE2 and infection.