Infection of Irf7−/− mice with the different JEV isolates and MVEV (n = 14 mice per group, n = 56 total infected mice) illustrated a mean weight loss of 5–10% for surviving mice across all four groups (Fig. 3C), with the exception of 20 mice that reached ethically defined endpoints for weight loss and/or disease manifestations (Supplementary Fig. 6). This evidence concerns the gene IRF7 and infection.