1. The expression level of cuproptosis gene in IDD was verified by in vitroand in vivoexperiments2. The results showed that the expression levels of FDX1, LIAS, LIPT1, GCSH, DLST, DLAT and PDHB in IDD samples were significantly decreased, while the expression levels of ATP7A, ATP7B and MTF1 were significantly increased. This evidence concerns the gene PDHB and intervertebral disk degenerative disorder.