Anti-COPT1-IgG and anti-COPT1-IgM autoantibodies can effectively distinguish NSCLC from NC with AUC values of 0.733 and 0.679 respectively, and distinguish NSCLC from BPN with AUC value of 0.648 and 0.571 respectively. The gene discussed is CD40LG; the disease is non-small cell lung carcinoma.