In addition, significantly higher levels of nutritional indicators (Hb, Alb, PA, TF and RBC) and immunoglobulin (IgG, IgA and IgM) were found in the observation group when compared with the control group (all p< 0.05); and the incidences of wound dehiscence and local skin necrosis in the observation group were remarkably lower than those in the control group (all p< 0.05). This evidence concerns the gene CD40LG and necrosis.