To further confirm that p50 K146ac contributed to the antitumor immune function of ACAT1 pS60, we induced Acat1-Flag WT or S58D expression in Nfkb1-depleted CT26 cells rescued with HA-mp50 (1-364aa) WT or K144R (equivalent to K146R in humans) and performed subcutaneous tumor model. The gene discussed is DDX53; the disease is neoplasm.