The immune-related functions of the two top-ranked metabolic enzymes—glutathione S-transferase mu 2 (GSTM2) and amine oxidase, copper containing-3 (AOC3)—have been widely discussed, indicating the reliability of our analytical method.32–35 However, the effect of the metabolic enzyme ACAT1 on the immune microenvironment in CRC remains unknown. This evidence concerns the gene ACAT1 and colorectal carcinoma.