Following the implantation of the E0771.LMB cells into Cyp11a1-mCherry reporter mice, we profiled the steroid hormones by LC-MS/MS, and isolated CD45+mCherry+ and CD45−mCherry− tumour-residing immune cells for in-depth scRNA-seq and flow cytometric assessments (Fig. 4A). Here, CYP11A1 is linked to neoplasm.