Inhibition of the AMPK/mTOR/ULK1 pathway (50–200 μM)Attenuated cell proliferation in both U251 and U87 cells in a dose-dependent manner (50–200 μM)Inducing the apoptosis (50–200 μM)Regulating the Bax, Cytochrome C, cleaved caspase-3Reducing the expression of Bcl-2 proteinsImpairing the migration and invasion in cells (50–200 μM)Decreasing the oxidative phosphorylation in berberin-treated U251 and U87 cellsReducing the glycolytic capacity in cells (50–200 μM)Inducing the autophagy in cells (50–200 μM)Reducing the tumor growth (at concentration of 100 μM). The gene discussed is CYCS; the disease is neoplasm.