MTOR and neoplasm: Inhibition of the AMPK/mTOR/ULK1 pathway (50–200 μM)Attenuated cell proliferation in both U251 and U87 cells in a dose-dependent manner (50–200 μM)Inducing the apoptosis (50–200 μM)Regulating the Bax, Cytochrome C, cleaved caspase-3Reducing the expression of Bcl-2 proteinsImpairing the migration and invasion in cells (50–200 μM)Decreasing the oxidative phosphorylation in berberin-treated U251 and U87 cellsReducing the glycolytic capacity in cells (50–200 μM)Inducing the autophagy in cells (50–200 μM)Reducing the tumor growth (at concentration of 100 μM)