Reduction in the tumor densityIC50 = 11.4 μM and 7.7 μM for C6 cells and C6-GSCs, respectively (in the presence of β-glucuronidase)Reduction in the colony formation in a time-dependent manner after prodrug 1b with β-glucuronidase and cyclopamine (at 10 μM)Max. reduction in colonies grown of about 50% (prodrug with β-glucuronidase) and 80% (cyclopamine) after 72 h of treatmentIncreasing the caspase-3/7 by 50 and 80% in C6 cells and C6-GSCs after treatment of 1b+β-glucuronidase, and by 100 and 130% with cyclopamine. The gene discussed is CASP3; the disease is neoplasm.