This study examined the effect of 4,4′‐diisothiocyanostilbene‐2,2′‐disulfonic acid (DIDS), an anion channel blocker of voltage‐dependent anion channel 1 (VDAC1), on noise‐induced hearing loss (NIHL) and its underlying mechanisms. The gene discussed is VDAC1; the disease is noise induced hearing loss.