Researchers who studied the association of environmental factors influencing gut microbiome and allergy response used a biochemical method, Phadiatop (ThermoFisher Scientific, Waltham, MA, USA), to check the Immunoglobulin E (IgE) concentration in the blood serum, which is an indicator for allergic response, in addition to microbial diversity [49,50]. The gene discussed is IGHE; the disease is Allergy.