The BCG cytoplasmic membrane is composed of a plasma membrane surrounded by lipid-attached peptidoglycan (PG) and arabinogalactan (AG), which are agonists of TLR2 and TLR4 and can be used as an adjuvant for tumor vaccines to enhance the maturation and migration of DCs in the tumor microenvironment. The gene discussed is TLR4; the disease is neoplasm.