Our study showed that the expression of the tBID gene did not compromise the replication capability of KD01 within bladder tumor cells and the modification of KD01 did not affect tumor cell death due to viral replication; however, tBID induced the formation of pores in the mitochondrial membrane, enhancing its permeability and elevating the apoptotic rate of the bladder cancer cells, particularly at the late stage of apoptosis. Here, BID is linked to urinary bladder neoplasm.