CD8A and central nervous system cancer: Immunohistochemistry (IHC) and immunofluorescent (IHF) staining of glioma tissue confirmed this increase and further revealed that CD8+T cells infiltrated deeper into the glioma tissue upon ITE treatment, as shown in Figure 2E. To assess the function of the tumor-infiltrated CD8+T cells, the level of IFN-γ was determined and found to be significantly increased in the PD1 and ITE+PD1 groups (Figure 2F).