Enzyme-linked immunosorbent assay (ELISA) was performed using sera from 230 normal individuals and 483 different cancer patients, including 96 HCC patients, and AEG-1 lung homing domain (381–443 a.a.)was used as an antigen, to detect the auto anti-AEG-1 antibody [147]. Here, MTDH is linked to hepatocellular carcinoma.