As 7aaRGD is expected to block SPP1/integrin signaling, we tested its efficacy in co-cultures of primary mouse microglia with U87-MG glioma cells, which were stably transfected with either a control shRNA (shNeg) or an SPP1-targeting shRNA to achieve SPP1 gene silencing (shSPP1). This evidence concerns the gene SPP1 and central nervous system cancer.