Notably, the transfection of either YAP1ΔWW (Figure S8A–D, Supporting Information) or His‐DNA‐PkcsΔKinase (Figure S8E–H, Supporting Information) was able to alleviate hyperglycemia‐induced ferroptosis and cardiomyocyte damage, underscoring the pivotal role that the DNA‐PKcs/YAP1 interaction plays in the modulation of hyperglycemia‐associated ferroptosis. The gene discussed is YAP1; the disease is Hyperglycemia.