However, the overexpression of NEDD4L C821A (an E3 ligase activity‐dead mutant) failed to decrease the proliferation of colorectal cancer cells, and the overexpression of NEDD4L R776Q (a constitutively active mutant) increased the suppressive effect of NEDD4L on the proliferation of colorectal cancer cells (Figure 2B,D). The gene discussed is NEDD4L; the disease is colorectal cancer.