Moreover, when PRMT5 was knocked out (Figure 6C–E) or when the PRMT5 PPNAA mutant, whose degradation cannot be mediated by NEDD4L, was overexpressed (Figure 6F–I), NEDD4L failed to inhibit the AKT/mTOR signaling pathway and had no effect on colorectal cancer cell proliferation. Here, PRMT5 is linked to colorectal cancer.