Initially, we subjected WT and Nf1 heterozygous (Nf1+/−) mice to oxygen-induced retinopathy (OIR; Figure 5A) to demonstrate that neurofibromin expression is reduced in WT retina lysates and that F4/80 expression, a putative macrophage marker, is increased in Nf1+/− retinas subjected to OIR (Figures 5B–5E). This evidence concerns the gene NF1 and retinal disorder.