To investigate the contribution of SMC4 to prostate cancer growth and metastasis, we inoculated RM1‐LM‐Vector cells and SMC4 knockdown cells into the tibia of C57BL/6 mice, and 14 days after injection, the mice inoculated with RM1‐LM‐Vector cells showed dramatic increases in bioluminescent imaging (BLI) signals compared with mice inoculated with SMC4 knockdown cells (Figure5A). This evidence concerns the gene SMC4 and Familial prostate cancer.