Regarding the Late-cfDNA-Gluc effect on ES-2 cells, TLR9 can underlie the cancer-favoring phenotypical changes observed, such as the metabolic remodeling and the pro-proliferation stimulus in unselected cells and the more prone quiescence induction in ES-2 cells long-term selected by this cfDNA variant, which ultimately supports chemoresistance in a cysteine-dependent manner. This evidence concerns the gene TLR9 and cancer.