To further explore the cytotoxicity of CD8+ T cells on day 7 after CTT, splenic CD8+ T cells from the tumor-bearing control and CTT-treated mice were isolated via magnetic-activated cell sorting (MACS) and cocultured with calcein-AM-labeled B16F10 tumor cells to detect calcein fluorescence in the supernatant. The gene discussed is CD8A; the disease is neoplasm.