Single-cell RNA sequencing (scRNA-seq) analysis conducted on patient-derived GBM neurospheres enriched with GSCs ± transgenic Oct4/Sox2 showed a shift towards the MES-like cell state and away from the OPC-like, NPC-like, and AC-like neurodevelopmental phenotypes (Figure 1B) [22]. The gene discussed is POU5F1; the disease is glioblastoma.