As VEGF and NRP1 expression in breast cancer were associated positively with lymph node metastasis, to understand the role of the VEGF/NRP1 axis in breast cancer, a stable VEGF-silencing Metastatic mammary adenocarcinoma cell lines (MDA-MB-231/shVEGF) cells and NRP1 over-expressing MCF-7/NRP1 cells were generated [137]. This evidence concerns the gene NRP1 and breast adenocarcinoma.